Measurement of ROS .... 3

Measurement of ROS .... 3







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Measurement of ROS .... 3
Posted in 2013

The antioxidant levels of the semen can also be determined by chemiluminescence assay or by a colorimetric assay. Antioxidant levels are measured through the addition of a known concentration of ROS to the semen, leading to the development of the chemiluminescence signal or a color change. This assay allows the antioxidants in the semen to scavenge the known ROS and then the measurement of residual ROS level. The intensity of the signal produced is inversely correlated with the total antioxidant capacity of the sample .
Another method for measuring oxidative stress can be carried through the measurement of lipid peroxidation in the whole sperm by a commercially assay kit (LP Sperm Test, Diacron International, Grosseto, Italy). The assay is based on the ability of peroxides to promote the oxidation of Fe2+ to Fe3+; the product of peroxidation (Fe3+) binds to the thiocyanate, developing a colored complex measured photometrically

My advise
1...  Oxygen is important for our life.   Without oxygen we cannot survive
2...  Oxygen is important for digesting our food
3...  Oxygen is important for our brain, lungs and kidney function,, cleaning our blood etc..
4...  Oxygen is important for sperm health of the male
5...  Follow healthy food for your body and sperm health

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